Here, the protective effects of dp extract against diabetesinduced renal fibrosis were evaluated. Moringa oleifera is a remarkably nutritious vegetable with several antioxidant properties. Characterisation of streptozotocin induced diabetes mellitus in swiss albino mice 11sachin arora, shreesh kumar ojha and divya vohora2 2department of pharmacology, faculty of pharmacy, jamia hamdard, new delhi, india abstract. Amelioration of streptozotocininduced diabetes mellitus, oxidative stress and. Diabetes was induced in male wistar rats by the administration of stz 60 mgkg b. In the present study an attempt has been made to evaluate the presence of hypoglycemic activity in the alcoholic extract of aloe vera gel. The objective of this study was to determine the effect of combined supplementation of pioglitazone and acacia. Hypoglycemic effect of carica papaya leaves in streptozotocin.
Ameliorative effects of glycyrrhizin on streptozotocin. After 5 days, dp extract 25 mgkgday and metformin 50 mgkgday were administered orally to diabetic rats for 28 days. Antihyperglycemic effect of ginkgo biloba extract in. Cessation of these preventive treatments was followed by the appearance of insulitis and diabetes. Dec 18, 2007 figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected. An international journal of pharmaceutical science on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Sd rats were used to induce diabetes using streptozotocin. Changes in the blood glucose levels in diabetic rats after crude polysaccharide and rhamnoseenriched polysaccharide treatment. Effect of supplementation of acacia honey and pioglitazone.
Streptozotocininduced diabetic models in mice and rats. I z zador, g d deshmukh, r kunkel, k johnson, n s radin, and j a shayman. Effect of phaseolus vulgaris on circulatory antioxidants. This study determined the profile of protein expression in the gastrocnemius muscle of rats with streptozotocininduced diabetes that were chronically exposed to f. Retinolbinding protein rbp and transthyretin ttr in the plasma, liver and kidney, retinol in plasma, and total vitamin a in the liver were measured in rats 6 weeks after diabetes mellitus had been induced by streptozotocin stz. The effect of phaseolus vulgaris, an indigenous plant used in ayurvedic medicine in india, on circulatory antioxidants and lipids was studied in rats with streptozotocin induced diabetes. To better understand the insulinindependent plasma glucoselowering action of metformin, we used streptozotocin stzinduced diabetic rats to investigate the possible mechanisms. The full text of this article is available as a pdf 180k.
Jan 17, 2018 increased reactive oxygen species ros generation in diabetes mellitus dm is an important mechanism leading to diabetic cardiomyopathy. Chronic hyperglycemia and the corresponding glucotoxicity are the main pathogenic. Full text streptozotocininduced type 1 diabetes in rodents. Insulin treatment was able to lower ischemic damage by 64% as compared with the diabetic group. Sep 21, 2017 in our study, the antihyperglycemic effect of mulberry leaves which mixed into the food was studied with streptozotocin stz induced diabetes rats, and the potential mechanisms were explored. Oral intake of metformin decreased the plasma glucose of stzinduced diabetic rats with a parallel increase of plasma.
Characterisation of streptozotocin induced diabetes mellitus. A role for glycosphingolipid accumulation in the renal hypertrophy of streptozotocininduced diabetes mellitus. Streptozotocin stz, 60 mgkg was injected intraperitoneally in rats to induce diabetes. Stz is most often used to induce diabetes in rats and mice. Streptozotocin stz is an antibiotic that produces pancreatic islet cell destruction and is widely used experimentally to produce a model of type 1 diabetes mellitus t1dm. The streptozotocin stz induced diabetic model is widely used. Streptozotocin injection produced significant changes in oxidative stress parameters in the serum of diabetic rats 4 weeks after diabetes induction, as shown by increased lipid peroxidation product mda and decreased sod, gst, and cat activities versus the control group p free access 10. The study investigates the effect of glycyrrhizin on streptozotocin stz.
Jan 17, 2020 changes in the blood glucose levels in diabetic rats after crude polysaccharide and rhamnoseenriched polysaccharide treatment. Propolis effect on streptozotocininduced diabetic rats. Leptin therapy reverses hyperglycemia in mice with. Clinical and experimental studies have demonstrated the promising protective effects of propolis in dm and its associated systemic effects. Streptozotocininduced diabetic neuropathy in mice and rats induction. Herein, we investigated the effect of malaysian propolis mp on. Induction of diabetes by streptozotocin in rats springerlink. Thus stz diabetes causes a significant decrease in cardiac contractile. In our experiments, we followed the rats with streptozotocin induced diabetes for one year and a recovery was observed in a significant portion of animals. Animals will be injected with streptozotocin stz ip or iv once after fasting for 1218hrs animals will have free access to food after stz administration. For this study, any diabetic rat that lost 10% of its initial body weight was treated with 12 units of insulin every other day in order to maintain weight.
Here, the protective effects of dp extract against diabetes induced renal fibrosis were evaluated. Streptozotocininduced diabetes in the rat is associated with. Studies of streptozotocininduced insulitis and diabetes. Pdf hypoglycemic effect of yacon tuber extract and its. Amelioration of streptozotocininduced diabetes mellitus, oxidative. Graviola annona muricata, a small tree of the annonaceae family, has been investigated for its protective effects against diabetic complications, oxidative stress, and neuropathies. A spontaneous recovery from streptozotocin induced diabetes was already described in neonatal rats, in which streptozotocin was applied at birth, garofano et al. Streptozotocin definition of streptozotocin by the free. Traditional plant treatment for diabetes has shown a surging interest in the last few decades. On the first day of the experiment, the diabetes induced groups of. Receive free emailalerts when new articles cite this article.
Objective the present study was designed to evaluate the diabetes induced neuronal damage in the hippocampus of the rat brain. Aging, urbanization, and an unhealthy lifestyle such as inadequate diet and sedentarism are mainly responsible for its increased incidence. Streptozotocin is a glucosaminenitrosourea compound. Despite numerous treatment options, the management of diabetes without side effects remain a big challenge. Hypoglycaemic activity of ethanolic extract of garcinia. The responses are accompanied by corresponding inverse changes in plasma insulin and sequential ultrastructural changes resulting in necrotic beta cell death. Reduced anterograde and retrograde accumulation of. Treatment of streptozotocininduced diabetic rats with ave7688, a. Elevations in plasma glucose and free fatty acids are thought to increase reactive. This study was planned to investigate the effects of graviola on behavioural alterations and.
Brazilian propolis, diabetes, glucose, free fatty acids. In an attempt to establish its medicinal value, the present study was carried out to determine the hypoglycaemic potential of g. Extract of moringa oleifera leaves ameliorates streptozotocin. Induction of diabetes by streptozotocin in rats ncbi nih. Diabetes was induced by stz injection into male, 6 to 23 weeks old, sprague dawley rats. Improving the reliability and utility of streptozotocininduced rat. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and cpeptide in serum, between normal and diabetic rats. The effect of streptozotocininduced diabetes in rats on. The effect of phaseolus vulgaris, an indigenous plant used in ayurvedic medicine in india, on circulatory antioxidants and lipids was studied in rats with streptozotocininduced diabetes. Administration of both streptozotocin stz and nicotinamide na has been proposed to induce experimental diabetes in the rat.
Several studies have reported that some parts of the c. Dietetic supplementation with branched chain amino acids. Pdf induction of diabetes by streptozotocin in rats researchgate. Pdf induction of diabetes mellitus in rats using intraperitoneal. Recurrent hypoglycemia exacerbates cerebral ischemic damage. In order to determine whether diabetic cardiomyopathy in rats is associated with altered contractile proteins, male and female rats were made diabetic with intravenous streptozotocin stz. After 5 days, dp extract 25 mgkgday and metformin 50 mgkgday were. The effects of 1 hemin, an inducer of ho expression and activity, and 2 zinc protoporphyrin ix znppix, an inhibitor of ho activity, have also been investigated on the tissue injury by ir and some.
Propolis effect on streptozotocininduced diabetic rats scielo. This study investigated the role of heme oxygenase ho1 in the cardiac tissue injury of acute ischemiareperfusion ir in diabetic streptozotocin stzinduced hyperglycemic rats. In our study, the antihyperglycemic effect of mulberry leaves which mixed into the food was studied with streptozotocin stzinduced diabetes rats, and the. Stz is well known to cause pancreatic bcell damage, whereas na is administered to rats to partially protect insulinsecreting cells against stz. The ginkgo biloba extract gbe has been reported to have a wide range of health benefits in traditional chinese medicine. Diabetes is associated with declining sexual function in women. Jan 14, 2011 objectives glycyrrhizin is the main water.
Previous studies showed that the diabetogenic action of streptozotocin is reduced in rats adapted to a highprotein, carbohydrate free diet, that have markedly elevated plasma concentrations of valine, leucine and isoleucine. Longterm streptozotocininduced diabetes in rats leads to severe. Streptozotocin diabetes induced by multiple low doses of the. Filtered tap water and rat chow pellets were available ad libitum. Streptozotocin stz induced diabetes mellitus dm offers a very cost effective and expeditious technique that can be used in most strains of rodents, opening the field of dm research to an array of genotypic and phenotypic options that would otherwise be inaccessible.
Oral administration of dahi containing probiotic lactobacillus acidophilus and lactobacillus casei delayed the progression of streptozotocininduced diabetes in rats volume 75 issue 2 hariom yadav, shalini jain, pushpalata ravindra sinha. Streptozotocininduced diabetic neuropathy in mice and rats. Streptozotocininduced type 1 diabetes in rodents as a model. The neurological consequences of diabetes mellitus in the brain have gained attention most recently. Among animal models of diabetes mellitus, streptozotocin rat was considered. Apocynin, a drug isolated from the herb picrorhiza kurroa, is considered an antioxidant agent by inhibiting nadph oxidase activity and improving ros scavenging. Effect of naringerin on biochemical parameters in the streptozotocin.
Medicinal plants attract growing interest in the therapeutic management of diabetes mellitus. Pdf effect of streptozotocin induced diabetes on rat. Mulberry leaf alleviates streptozotocininduced diabetic. Detailed in this unit are protocols for producing stz.
Yacon is potentially beneficial for the management of diabetes and is composed of fructooligosaccharides, proteins, minerals and phenolic compounds. This study investigated the role of heme oxygenase ho1 in the cardiac tissue injury of acute ischemiareperfusion ir in diabetic streptozotocin stz induced hyperglycemic rats. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water. In control and streptozotocin treated female rats, we investigated the effects of diabetes on vaginal blood flow, tissue morphology, and expression of arginase i, endothelial nitric oxide synthase enos and cgmp.
Streptozotocininduced diabetes in the rat is associated. Hypoglycemic effect of aloe vera gel on streptozotocin. Male wistar rats 60 days old were randomly distributed into two groups of 18 animals. Antidiabetic activity of crude polysaccharide and rhamnose. Diabetes mellitus dm is considered an epidemic disease causing great challenge to public health all over the world.
Nov 28, 2012 data are preliminary on the hypoglycemic effect of carica papaya leaves in streptozotocin induced diabetic rats. Streptozotocininduced diabetes lowers retinolbinding. The aim of this study was to evaluate the protective effects of d. There is growing interest in the use of alternative approaches like the use of herbs and natural products in the management of diabetes. As with other alkylating agents in the nitrosourea class, it is toxic to cells by causing damage to the dna, though other mechanisms may also contribute. Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with nonketosis. There is a lack of appropriate animal model that could spontaneously develop dn. The experimental diabetes was induced in rats by a single dose of stz 40 mgkg i. Hypoglycemic and hypolipidemic effect of seed hydromethanolic extract of tamarindus indica l. Pdf propolis is one of the hive products that has been used extensively in.
The present study was designed to investigate the characteristics of diabetes mellitus induced by. Stz is well known to cause pancreatic bcell damage, whereas na is ad. Intravenous injection of 60mgkg dose of streptozotocin in adult wistar rats, makes pancreas swell and at last. Evaluation of antidiabetic property on streptozotocininduced.
In control and streptozotocintreated female rats, we investigated the effects of diabetes on vaginal blood flow, tissue morphology, and expression of arginase i, endothelial nitric oxide synthase enos and cgmp. Mulberry leaf alleviates streptozotocininduced diabetic rats. The effect of streptozotocininduced diabetes in rats. As expected, uncontrolled diabetes streptozotocin diabetes, untreated animals resulted in a 70% increase in ischemic damage as compared with the control group. Pdf propolis effect on streptozotocininduced diabetic rats. In order to test the role of these branched chain amino acids bcaa in the beneficial effects of the highprotein diets, rats adapted 15 days either to a balanced.
Influence of apocynin on cardiac remodeling in rats with. Stzdiabetic rats n96 showed stable signs of diabetes hyperglycaemia. Therefore, the current research study was directed toward the identification of the chronic antioxidant and antidiabetic properties of an aqueous extract of m. Rabbit antimouse lymphocyte serum, alone, provided partial protection but, when given together with either 3omg or nicotinamide, effectively prevented the streptozotocininduced diabetic syndrome. The present study assessed the possible antioxidant and antidiabetic effects of an aqueous extract of m. Streptozotocin use in mice and rats version 1 date of publication. Streptozotocin stz is a widely used chemical for the induction of experimental diabetes in rodents. Induction of experimental diabetes mellitus is indeed the first step in the plan of purification of pancreatic langerhans islet cells of normal rats for transplanting under the testis subcutaneous of experimentally induced diabetic rats. Therefore, the purpose of this study was to assess the hypoglycemic effect of the aqueous extract of c.
In this study, diabetes was induced by a single injection of streptozotocin stz, 45 mgkg, i. Stz diabetic rats were treated with oral doses of mebs 200 and 400 mgkg for 8 weeks. The aim of this paper is to describe a step by step protocol on how to induce diabetes type 2 in rats using streptozotocin stz nicotinamide na. A spontaneous recovery from streptozotocininduced diabetes was already described in neonatal rats, in which streptozotocin was applied at birth, garofano et al.
The diabetic rats gained 83% less weight despite consuming 45% more feed than the nondiabetic controls. The diabetesinducing agent streptozotocin stz is a. Background diabetes mellitus is an endocrine metabolic disorder of impaired carbohydrate, fat and protein metabolisms characterized by chronic hyperglycemia. Furthermore, central delivery of leptin was found by hidaka et al. Neuron cells and blood vessels in the dm rat brains became. Read ameliorative effects of glycyrrhizin on streptozotocin. Hyperglycemia in streptozotocininduced diabetic rat. Proteomic analysis of gastrocnemius muscle in rats with. An antibiotic, c8h15n3o7, produced by an actinomycete, used primarily to treat pancreatic tumors affecting the islets of langerhans. Oxidative stress, inflammation and apoptosis are major complications that trigger organ failure in diabetes mellitus dm, and are proven to adversely affect the male reproductive system. Oxidative stresses intensify the progression of diabetesrelated behavioural changes and testicular injuries. Pdf the objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal.
To understand the pathophysiological mechanisms and factors involved in diabetes, the use of pancreatic regeneration studies is increasing in an attempt to understand the behavior of pancreatic beta cells. This project has assessed cardiovascular, ocular and neuropathic changes over a period of 24 weeks poststz treatment in rats. The longterm effects of streptozotocin and possible recovery from streptozotocin induced diabetes are explored much less. Effect of phaseolus vulgaris on circulatory antioxidants and. In one group, diabetes was induced through the administration of streptozotocin. Recurrent hypoglycemia exacerbates cerebral ischemic. Dna damage induces activation of parp which is likely more important for diabetes induction than the dna damage itself. The aim of this study was to investigate the hypoglycemic effect of yacon tuber extract yte and its constituent, chlorogenic acid cga, in streptozotocin stzinduced diabetic rats. However, the effects of diabetes on genital tissue structure, innervation and function remains poorly characterized. Type 1 diabetes t1d is a pathological condition characterized by the loss of insulinproducing. Pdf induction of diabetes by streptozotocin in rats. The aim of this study was to evaluate the antihyperglycemic effects of gbe on streptozotocin stz induced diabetes in rats.
Streptozotocininduced type 1 diabetes in rodents as a model for studying mitochondrial mechanisms of diabetic. Role of insulin, oxidative stress and nitric oxide nagy m a1, bastawy m a1, 2abdelhamid n m, 1chemistry department, faculty of science, benisuef university, egypt 2biochemistry department, faculty of pharmacy, miniauniversity, egypt. Hypoglycemic and hypolipidemic effect of seed hydromethanolic. Diabetesinduced hyperglycemia is related to damage in several organs and associated with vascular and cardiac diseases such as. Calcium atpase activity of cardiac actomyosin was significantly decreased after 1 week of diabetes and was depressed by 60% by 2 weeks. There were deficits in accumulation on both sides of the constriction in untreated diabetic rats proximal accumula tion 66% of controls, p streptozotocin induced animal models of diabetes.
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